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Quantitation of abLIM binding to actin filaments. Assay mixtures containing different total amounts of GST–abLIM polypeptide (putative actin-binding domain of abLIM, amino acids 585–778) were processed to separate bound abLIM in each pellet fraction (P) from free abLIM in the supernatant fractions (S). The derived values are plotted in a. The total amount of abLIM added (μg) in each 60-μl reaction mixture is indicated above each pair of gel lanes in b.
