Figure 5. Evidence for expression of MCOX2 in male gonads from nine unionoidean bivalve genera.
Evolutionary tree depicting species relationships and maximum likelihood-based patristic distances, using Fcox1 DNA sequences, among the nine genera represented in this study (tree after Campbell et al. [24]). Western blot analysis lanes are presented after the species names and show MCOX2 expression in male gonads representing seven genera. In Fusconaia and Plethobasus, MCOX2 expression is evidenced by IEM results. The divergence of these genera occurred over an evolutionary history >65 myr. Equal amounts of protein were loaded in all lanes.