Humoral immune responses of adult female tree swallows as a function of age. (a) Acquired humoral response to SRBC challenge (SRBC Ab titre; r=−0.273, p=0.120, n=33). Note: 1 was added to the argument of the logarithm to show titres=0 corresponding to undiluted plasma, (b) levels of natural antibodies (NAbs titre; r=0.111, p=0.531, n=34), (c) complement-mediated cell lysis (Lysis titre; Spearman r=0.065, p=0.724, n=32).