Figure 6.

Tiam1 overexpression results in increased adhesion to laminin. N1E-115 cells stably expressing C1199 Tiam1 or a control cell line were plated on 24-well plates coated with increasing concentrations of laminin. A small portion of the cells was pretreated in suspension with DME/1% BSA in the presence of 10 μg/ml of affinity-purified anti-α6 antibody (GoH3), or mock treated, and plated in wells coated with 20 μg/ml laminin. Cells were allowed to attach for 15 min, washed, and adhesion was quantitated by crystal violet staining and measuring absorbance at 570 nm (for details see Materials and Methods). Maximal adhesion was determined on poly-l-lysine–coated dishes and set at 100%. Values represent the average of quadruplicate samples from two representative experiments. Error bars indicate SEM (n = 2). The observed differences at laminin concentrations of 20 and 10 μg/ml were found statistically significant by students t test (P < 0.01).