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. 1997 Nov 3;139(3):759–771. doi: 10.1083/jcb.139.3.759

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(A) NIH3T3-NEO or NIH3T3 cell lines transfected with bcl-2 were grown for 48 h in 0.5% FCS and incubated in serum-free medium (relative numbers and percentages of viable cells were determined by trypan blue dye exclusion). Data represent arithmetic means ± SD for three independent experiments. (B) Growth-arrested NIH3T3-NEO and NIH3T3–bcl-2 cells were incubated in serum-free medium for the indicated times. Lysates from both adherent viable cells and nonadherent apoptotic cells were combined and Western blot analysis was performed.