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. 1997 Nov 3;139(3):665–673. doi: 10.1083/jcb.139.3.665

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Lysosome–lysosome fusion in vivo. To determine if lysosome–lysosome fusion occurred in vivo, alveolar macrophages were loaded with either 1 mg/ml FITC-dextran or 0.5 mg/ml Texas red–dextran at 37°C for 30 min. Cells were washed and incubated for an additional 90 min in HMEM. Cells were then fused using UV sendai virus protocols as described in Materials and Methods. Cells were washed, plated onto glass coverslips, and then placed back in HMEM at 37°C for various times and examined by fluorescence microscopy.