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. 1997 Nov 3;139(3):785–795. doi: 10.1083/jcb.139.3.785

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Colocalization of AF-6 with ZO-1 in Rat1 and PC12 cells. Subconfluent Rat1 (a–c) and PC12 (d–f) cells were doubly stained with a rabbit polyclonal antibody against AF-6 (a, c, d, and f) and a mouse monoclonal antibody against ZO-1 (b, c, e, and f), followed by FITC-conjugated anti–rabbit IgG and Texas red-conjugated anti–mouse IgG antibodies, and examined using laser scanning confocal microscopy. AF-6 is shown in green (a, c, d, and f) and ZO-1 (b, c, e, and f) is shown in red. In images c and f, the yellow area indicates the colocalization of AF-6 and ZO-1. The results shown are representative of three independent experiments. Bars: (a–c) 10 μm; (d–f) 5 μm.