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. 1997 Nov 3;139(3):785–795. doi: 10.1083/jcb.139.3.785

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Interaction of recombinant AF-6 with ZO-1. Crude lysates of Sf-9 cells infected with baculovirus carrying HA-AF-6 cDNA were loaded onto affinity columns immobilized with GST (lane 1), GST-ZO-1 (lane 2), GST-occludin (lane 3), and GST-CD44 (lane 4). The interacting proteins were eluted with GST fusion proteins by the addition of glutathione. The eluates were subjected to SDS-PAGE and followed by immunoblot analysis with anti–AF-6 antibody. The arrowheads denote the positions of HA-AF-6. The results shown are representative of three independent experiments.