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. 1997 Nov 3;139(3):625–638. doi: 10.1083/jcb.139.3.625

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7B2-induced activation of proPC2 is pH and calcium dependent. (a) Golgi-enriched membranes were incubated at different pH at 37°C in the presence of 200 nM recombinant 21-kD 7B2. The reaction was stopped by the addition of Laemmli sample buffer and boiled for 5 min before PC2 immunoblotting. (b) Golgi-enriched membranes were incubated in the presence or absence of 200 nM recombinant 21-kD 7B2 (21 kD) and of 5 mM calcium at 37°C for 30 min. When calcium was omitted, it was replaced by 2 mM EDTA. The reaction was stopped by the addition of Laemmli sample buffer and boiled for 5 min before PC2 immunoblotting. Alternatively, the same incubations were performed to measure PC2 activity. Activity measurements are presented on the bottom line under the immunoblots as percent of the maximum activity detected.

7B2-induced activation of proPC2 is pH and calcium dependent. (a) Golgi-enriched membranes were incubated at different pH at 37°C in the presence of 200 nM recombinant 21-kD 7B2. The reaction was stopped by the addition of Laemmli sample buffer and boiled for 5 min before PC2 immunoblotting. (b) Golgi-enriched membranes were incubated in the presence or absence of 200 nM recombinant 21-kD 7B2 (21 kD) and of 5 mM calcium at 37°C for 30 min. When calcium was omitted, it was replaced by 2 mM EDTA. The reaction was stopped by the addition of Laemmli sample buffer and boiled for 5 min before PC2 immunoblotting. Alternatively, the same incubations were performed to measure PC2 activity. Activity measurements are presented on the bottom line under the immunoblots as percent of the maximum activity detected.