Table I.

Secretion of Newly Synthesized and Sulfated 7B2 and ProPC2

Time				Percent secreted
				30 min		60 min
min
Methionine		7B2		10		27
		PC2		0.3		15
Sulfate		7B2		29		—
		PC2		31		—

AtT-20 cells were labeled for 10 min with [³⁵S]methionine and cystine (Methionine), or for 15 min with [³⁵S]sulfate (Sulfate). They were then chased for the indicated time periods. 7B2 and PC2 were immunoprecipitated and separated by SDS-PAGE. The radioactivity in the gels was analyzed with a phosphorimager. The percent of 7B2 and PC2 released per total labeled 7B2 and PC2, respectively, was calculated in duplicate and the experiments were reproduced twice.