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. 1997 Nov 3;139(3):639–649. doi: 10.1083/jcb.139.3.639

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Quantitative analysis and graphical representation of Class II, DM, YAe, I-chain COOH terminus (IC), and I-chain NH₂ terminus (IN) distribution in endocytic compartments of 6H5.DM (A) and A20.A^b (B) cells. Ultrathin cryosections of 6H5.DM cells were single immunolabeled for the indicated molecules using 10-nm gold particles. For each immunolabeling, 20 cell profiles were randomly selected and gold particles were counted directly in the microscope. (A) In 6H5.DM cells, six types of endocytic compartments could be distinguished, and only these were subjected to quantitation. These compartments were identified by their morphological characteristics, as shown in the bottom of the figure. The percentages of gold particles present in each compartment are summarized in the table. The total number of gold particles counted is shown in the last column of the table. Percentages of labeling for DR, DM, and I-A^b (top graph) and of YAe, I-chain COOH terminus, and I-chain NH₂ terminus (bottom graph) are shown on the y axes. The graphs show strong enrichment of IC labeling in type 3 compartments, whereas labeling for DR, DM, and I-A^b is most abundant in types 5 and 6. IN labeling in types 3 and 4 is almost similar, while YAe is detected first in type 3 compartments and significantly enriched in the later types 4 to 6 (B). Quantitative analysis of A20.A^b cells was performed as described for 6H5.DM cells. I-chain NH₂ terminus is strongly enriched in type 3 compartments, and both class II and DM are abundant in type 4 and 5/6 compartments.

Quantitative analysis and graphical representation of Class II, DM, YAe, I-chain COOH terminus (IC), and I-chain NH₂ terminus (IN) distribution in endocytic compartments of 6H5.DM (A) and A20.A^b (B) cells. Ultrathin cryosections of 6H5.DM cells were single immunolabeled for the indicated molecules using 10-nm gold particles. For each immunolabeling, 20 cell profiles were randomly selected and gold particles were counted directly in the microscope. (A) In 6H5.DM cells, six types of endocytic compartments could be distinguished, and only these were subjected to quantitation. These compartments were identified by their morphological characteristics, as shown in the bottom of the figure. The percentages of gold particles present in each compartment are summarized in the table. The total number of gold particles counted is shown in the last column of the table. Percentages of labeling for DR, DM, and I-A^b (top graph) and of YAe, I-chain COOH terminus, and I-chain NH₂ terminus (bottom graph) are shown on the y axes. The graphs show strong enrichment of IC labeling in type 3 compartments, whereas labeling for DR, DM, and I-A^b is most abundant in types 5 and 6. IN labeling in types 3 and 4 is almost similar, while YAe is detected first in type 3 compartments and significantly enriched in the later types 4 to 6 (B). Quantitative analysis of A20.A^b cells was performed as described for 6H5.DM cells. I-chain NH₂ terminus is strongly enriched in type 3 compartments, and both class II and DM are abundant in type 4 and 5/6 compartments.

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