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. 1998 Feb 23;140(4):751–765. doi: 10.1083/jcb.140.4.751

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Analytical fractional of all family members in homogenates from rat liver and HeLa cells. Total membranes from rat liver (A) or HeLa cells (B) were centrifuged on linear sucrose (rat) or Nycodenz (HeLa) gradients as described in Materials and Methods. Equal volumes of each fraction were determined for their content of calnexin, Man II (Rat) or GalT (HeLa), syntaxin 5, p58 (rat), p53 (HeLa), and different p24 family members, using specific antibodies generated to the peptides described in Fig. 2 A and with antibodies specific to the Golgi markers (Man II, Gal T), the intermediate compartment markers (p58, p53) or ER marker (calnexin). The high density end of the gradient is on the right.