Figure 5.
DLP1 is associated with numerous punctate vesicular structures in cultured mammalian cells. Immunofluorescence microscopy of two distinct cultured cell lines, human fibroblasts (A) and a rat hepatocyte cell line, clone 9 (B), stained with affinity-purified antibodies to DLP1 (DLP-N). DLP1 is associated with numerous vesicular structures that are distributed throughout the cytoplasm and appear aligned along cytoskeletal filaments that extend outward from perinuclear foci (arrows). Higher magnification images of boxed regions in A and B are shown in A′ and B′, respectively, and strongly suggest a vesicular association. N, nucleus. Bar, 10 μm.