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. 1998 Feb 23;140(4):861–871. doi: 10.1083/jcb.140.4.861

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PI-3 kinase is not required for TCR-mediated MTOC reorientation in Jurkat T cells. Jurkat T cells were incubated with the indicated concentrations of Wortmannin for 30 min. Subsequently, Leu4-coated latex beads were added at a 1:2 ratio and conjugates were incubated for 30 min. Conjugates were fixed and stained with antitubulin antibody. The average of four independent experiments is shown.