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. 1998 Feb 23;140(4):861–871. doi: 10.1083/jcb.140.4.861

Figure 8.

Figure 8

Expression of dominant-negative ZAP-70 mutant impairs ITAM-mediated MTOC reorientation. Jurkat T cells were cotransfected with TT-εT or TT-ε (30 μg), together with 40 μg of empty vector or plasmids encoding different ZAP-70 mutants. 36 h later, cells were mixed with anti–Tac latex beads and incubated for 10 min at 37°C. Conjugates were fixed in paraformaldehyde and stained with antitubulin antibody. Percent MTOC inhibition equals X−N/M−N, where X is the percent MTOC reorientation triggered by TT-ε in the presence of dominant-negative ZAP-70, M is the percent MTOC reorientation triggered by TT-ε alone, and N is the percent MTOC reorientation triggered by TT-εT. Results are the average of three independent experiments. The averaged percent MTOC reorientation for each condition was 20 ± 6% for TT-εT plus vector, 67 ± 1% for TT-ε plus vector, 40 ± 6% for TT-ε plus SH2 (N+C), and 64 ± 5% for TT-ε plus SH2 (N+*C).