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. 1998 Feb 23;140(4):795–806. doi: 10.1083/jcb.140.4.795

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Caveolin-1 and -2 in MDCK cells. Anti–caveolin-1 immunoprecipitates were analyzed by 13% SDS-PAGE (A) and by 2D gel electrophoresis (B). For comparison, 2D gels of immunoisolated basolateral (C) and apical (D) vesicles are shown. α– and β–caveolin-1 are marked by arrowheads. Caveolin-2 is marked by arrows. Filter-grown MDCK cells metabolically labeled with [³⁵S]methionine were lysed and then caveolin was immunoprecipitated using anti– caveolin-1 amino-terminal antibodies. The samples were boiled in alkaline sample buffer before electrophoresis.