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. 1998 Feb 23;140(4):795–806. doi: 10.1083/jcb.140.4.795

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Caveolin-2 forms high molecular weight complexes. Western blotting using anti–caveolin-1 antibody N-20 or anti– caveolin-2 antibodies was performed after separating MDCK cell lysate on SDS-PAGE without boiling (A). Alternatively, the cell lysate was first fractionated by sedimentation velocity in a 5–30% sucrose gradient, and TCA proteins precipitated from the fractions were separated by 13% SDS-PAGE after boiling in alkaline sample buffer (B). The migration of molecular mass standards in the gradient is indicated.

Caveolin-2 forms high molecular weight complexes. Western blotting using anti–caveolin-1 antibody N-20 or anti– caveolin-2 antibodies was performed after separating MDCK cell lysate on SDS-PAGE without boiling (A). Alternatively, the cell lysate was first fractionated by sedimentation velocity in a 5–30% sucrose gradient, and TCA proteins precipitated from the fractions were separated by 13% SDS-PAGE after boiling in alkaline sample buffer (B). The migration of molecular mass standards in the gradient is indicated.