Table I.
Quantitative Analysis of Caveolin Distribution in Exocytic Vesicles from MDCK Cells
cav-1 | cav-2 | cav-1/cav-2 | ||||
---|---|---|---|---|---|---|
HA | ||||||
10.0 ± 0.9 | 11.0 ± 0.8 | 0.5 ± 0.2 | 19 ± 4 | |||
VSV-G | ||||||
3.8 ± 0.4 | 6.9 ± 0.7 | 4.0 ± 0.6 | 1.6 ± 0.2 |
The table shows one representative experiment. Routinely, gold label on 40 HA and 40 VSV-G–positive vesicles was quantitated. The number of gold particles counted per vesicle is shown. The ratio of label for caveolin-1 and -2 was calculated for each individual vesicle and is listed under cav-1/cav-2. Standard errors are given.