Table I.

Fenestrae Induction in Cocultures

Culture		[Fenestrae + clustered vesicles]/cell (mean)		SD
ACE		0.0189		0.0033
ACE + EPH4		0.0229		0.0042
ACE + EPH4/VEGF 164		0.1231		0.0500
ACE + EPH4/VEGF 120		0.1444		0.0632

Endothelial fenestrations and clustered diaphragmed vesicles in ACE cells in coculture with mouse mammary epithelial cells. Diaphragmed fenestrations and clustered vesicles were counted in ∼330 (ACE), 400 (ACE + EpH4), 390 (ACE + EpH4/ VEGF164), and 540 cells (ACE + EpH4/VEGF120) using sections from two independent experiments. Results were obtained from three section levels cut at a distance of 30 μm as described in Materials and Methods. The ratios of diaphragmed clustered vesicles and fenestrae number per cell profile were then determined. Student's t test was applied for pairwise comparison of the data and showed P < 0.001 for ACE versus ACE + EpH4/VEGF164; P < 0.005 for ACE versus ACE + EpH4/VEGF120; and P < 0.05 for ACE + EpH4 versus ACE + EpH4/VEGF120.