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. 1998 Feb 23;140(4):821–829. doi: 10.1083/jcb.140.4.821

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Spot photobleaching of GFP in the mitochondrial matrix. (A) Confocal fluorescence micrograph of CHO cells expressing GFP in mitochondria (100× objective, NA 1.4). (B) Photobleaching of GFP in the mitochondrial matrix of indicated cells. Measurements were done using a 100× objective (0.8-μm-diam spot) and 40 μs bleach time at 23°C. Each curve is the average of 10–15 fluorescence recovery curves from different cells. Bar, 5 μm.