Figure 5.
Microinjection of anti–cytochrome c mAb 2G8.B6 inhibits SCG apoptosis. SCG neurons were microinjected with either 20 mg/ml 2G8.B6 mAb (lane 1) or an equivalent concentration of mouse Ig (lane 2). After 2–4 h, the injected cells were counted, and the cultures were withdrawn from NGF. At 48 h, the number of surviving injected cells were counted and expressed as a percentage of the cells initially surviving injection. 150–200 cells were injected per coverslip, and the results shown are the average of five experiments. The error bars represent SEM.