Figure 7.

Coinjection of dATP does not enable cytochrome c to initiate apoptosis in SCG neurons. SCG neurons were microinjected with cytochrome c and dATP, counted 2–4 h later, and maintained in NGF for a further 72 h. The microinjection mix in the first four bars contained 0.7 mg/ml cytochrome c. dATP was added in the second bar at 100 μM, in the third bar at 2.5 mM, and in the fourth bar at 10 mM. The fifth bar contained 10 mM dATP alone, and the sixth bar contained no cytochrome c or dATP. The results are expressed as a percentage of the cells initially surviving injection. 150–200 cells were injected per coverslip, and the results shown are the average of three experiments. The error bars represent SEM.