Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Sep 21;142(6):1559–1569. doi: 10.1083/jcb.142.6.1559

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Cdc25 undergoes phosphorylation of Ser-287 and binds to 14-3-3ε in Xchk1-depleted extracts. (A) The 14-3-3ε protein binds to Cdc25 in Xchk1-depleted extracts. The 14-3-3ε protein was immunoprecipitated from a mock-depleted extract (lane 1), an Xchk1-depleted extract (lane 2), or an Xchk1-depleted extract supplemented with 2 ng/μl His6–Xchk1 protein (lane 3). For lanes 4–7, an M phase extract (lane 4) or interphase extracts (lanes 5–7) containing 1,000 sperm nuclei/μl (lane 5), 3,000 sperm nuclei/μl and 100 μg/ml aphidicolin (lane 6), or 3,000 UV-damaged sperm nuclei per μl (lane 7) were immunoprecipitated with anti–14-3-3ε antibodies. All extracts contained 100 μg/ml cycloheximide. In all cases, the anti–14-3-3ε immunoprecipitates were subjected to SDS-PAGE and immunoblotting with anti-Cdc25 (top) or anti–14-3-3ε (bottom) antibodies. APH, aphidicolin. (B) Mock-depleted extracts (lanes 1 and 2) and Xchk1-depleted extracts (lane 3) containing 1,000 sperm nuclei/μl were assayed for Ser-287–specific kinase activity using either GST–Cdc25(254– 316)-WT (lanes 2 and 3) or GST–Cdc25(254–316)-S287A (lane 1) as the substrate.