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. 1998 Sep 21;142(6):1501–1517. doi: 10.1083/jcb.142.6.1501

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Rotating images of 3-D reconstructions of the actin cytoskeleton. (A) A small-budded cell. (B) A budding zygote. Lines point to features of interest. 1, patches that overlap at certain angles. 2, an elongated patch that appears round at a certain angle of view. 3, a cable of uneven thickness along its length. 4, round patches at the ends of thin cables. 5, an elongated patch at the end of a thick cable. 6, a cable in the bud ending on a patch. 7, a cable end that is free but appears to overlap with a patch at certain angles of view. 8, a cable, both ends of which are not associated with a patch. These frames are sequential 2-D projections of rotating images of a vegetative cell and of a zygote with a step size of 24°. Angles of view are marked. The full data set in each case contains 180 frames with sequential projections at 2° angles. The movies are available at the authors' Web site, www.cooperlab.wustl.edu. Cells were stained with rhodamine-phalloidin. Deconvolution was with the EM method. Bar, 10 μm.