Figure 4.
Distribution of annexin XIIIb on exocytic TGN-derived carriers. (A) Immunostaining of HA (15 nm gold), annexin XIIIb (10 nm gold) and VIP21/caveolin-1 (5 nm gold) on TGN-derived carriers obtained from cells whose apical membrane have been ripped off (see Materials and Methods). (B) Immunostaining of VSV G (15 nm gold), annexin XIIIb (10 nm gold) and VIP21/ caveolin-1 (5 nm gold) on TGN-derived carriers. Bar, 100 nm. Note the enrichment of gold particles associated with annexin XIIIb on HA-containing carriers versus the amount observed on VSV G-containing carriers and see Table II. Bar, 100 nm.