Skip to main content
. 1998 Sep 21;142(6):1571–1581. doi: 10.1083/jcb.142.6.1571

Figure 7.

Figure 7

Association of 270-kD ankyrinG to the plasma membrane of human kidney 293 cells requires the membrane-binding domain and cotransfected neurofascin. Transfected 270-kD ankyrinG (Ank270-GFP) alone visualized by the GFP fluorescence distributes throughout the cytoplasm of 293 cells (A). Cotransfection with neurofascin (HA–NF) recruits Ank270–GFP to the plasma membrane (C). (B) Double labeling of cotransfected neurofascin by immunostaining of the HA epitope. The membrane-binding domain of ankyrin (M-GFP) is sufficient to be recruited to the plasma membrane (E) by cotransfected neurofascin (D). Deletion of the first half of the membrane-binding domain (Ank270(ΔM1,2)–GFP) abolishes the membrane-recruitment by cotransfected neurofascin (F and G). Bar, 3 μm.