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. 2007 Nov 14;104(47):18439–18444. doi: 10.1073/pnas.0707292104

Fig. 2.

Fig. 2.

The H3K27 demethylase activity of UTX requires a catalytically active JmjC domain. (A) The TPR domains are required for the optimal demethylase activity of UTX on H3K27me1 but are dispensable for UTX activity on H3K27me2/3. (Upper) Schematic representation of full-length and 401-1401 aa fragment of human UTX protein. The location of the conserved Fe(II)-binding H1146 within JmjC catalytic domain is indicated. (Lower) Plasmids expressing FLAG-tagged UTX, either full-length or 401-1401 aa fragment, were transfected into 293T cells followed by protein isolation and histone demethylase assay on calf core histones as described in Fig. 1. IP, immunoprecipitation. (B) Mutation of the Fe(II)-binding H1146 in the JmjC domain abrogates the H3K27 demethylase activity of UTX. Plasmids expressing FLAG-tagged wild-type or H1146A mutant UTX were transfected into 293T cells followed by protein isolation and histone demethylase assay as described above.