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. 2007 Nov 13;104(47):18445–18450. doi: 10.1073/pnas.0706784104

Fig. 4.

Fig. 4.

Nucleotide binding site. (A) View of the Ss-aIF2α3βγ nucleotide-binding pocket showing the positions of relevant aIF2β and aIF2γ residues, as discussed in the text. The composite annealed 2Fo − Fc omit map was contoured at 1 σ. (B) View of the Ss-aIF2α3β1γ nucleotide-binding pocket, drawn in approximately the same orientation as in A. The composite annealed 2Fo − Fc omit map was contoured at 1 σ. Comparison of A and B shows the differences between the GDP–Pi and the GDP–Mg2+ states. (C) Close-up stereoview of the putative phosphate-binding pocket. The side chains of relevant residues are drawn. The water molecule linking the phosphate group to the NH of g-G96 is drawn as a red sphere. Hydrogen bonds surrounding the Pi group are shown. The 2Fo − Fc map contoured at 1 σ (firebrick) and the Fo − Fc map contoured at 4 σ (blue) were calculated before the addition of the Pi and water molecules in the model. B factors (in Å2) of the atoms coordinating the phosphate (72 Å2) and the water (29 Å2) are as follows: 34 (NH g-G96), 52 (Nδ g-H97), 55 (Nε g-K48), and 63 (Nδ g-H37).