Table 1.

Crystallographic and refinement data

Parameter	Crystal
	α3β1γ:GDP-Mg2+	α3βγ:GDP-Pi
Data collection wavelength, Å	0.9795	1.007
X-ray source	id14eh4	id23eh1
Space group	C2	P3221
Cell parameters
a/b/c, Å	122.1/51.8/98.0	118.6/118.6/160.9
β, °	94.4	—
Unique reflections	31,835	21,823
Resolution, Å	2.15	3.2
Completeness, %	95.3	98.7
Redundancy	2.3	4.3
Rsym (I),* %	5.2 (32.0)	11.1 (33.8)
R/RFree,† %	21.7/26.4	25.4/31.4
rmsd bonds, Å	0.0067	0.0089
rmsd angles, °	1.337	1.708
Residues in model
γ	2–34; 49–340; 348–415; GDP-Mg2+	2–415; GDP, Pi
β	2–19	3–22; 28–139; Zn
α3	176–265	174–266
Mean B values
γ	47	57
β	62	64
α3	38	113
GDP	59	67
Pi	—	72
Mg2+	51	—
Zn (β subunit)	—	58
Water	48‡	29§

Ramachandran plots are shown in SI Fig. 10.

*Values in parentheses are R_sym (I) in the highest shell of resolution.

^†R_free was calculated with 6% of the reflections.

^‡Included in the model were 253 water molecules.

^§This value corresponds to the B value of the Pi bound water molecule.