Figure 4.

BVP does not have nucleotide phosphohydrolase activity. [α-³²P]ATP was incubated for 10 min at 30°C either with buffer (lane 1), the indicated amounts of BVP (lanes 2–4), C. elegans capping enzyme triphosphatase (Cel-1[1–236], lanes 5–7), or 1 unit of CIP (lane 8). The reaction mixtures were analyzed by TLC on PEI–cellulose plates. The positions of authentic ADP and AMP markers were detected by UV light.