Fig. 3.
Conditional loss-of-function of β-catenin affects SHF formation. (A) Scheme of mouse embryo indicates SHF in green with a transverse section plane at E8.5. (B–K) Whole-mount in situ hybridization for Isl1, dHand, Fgf8, and Fgf10 in controls (B, F, J, and L) and MesP1-cre; β-cateninlox/lox embryos (C, G, I, and K) at the eight-somite stage and for Wnt11 (compare control in D with mutant in E) at the 10-somite stage. (B1–C4 and F1–G2) Transverse sections in four section planes (indicated with dotted lines in B and F). (B1 and C1) Pharyngeal arch. (B2, C2, F1, and G1) Outflow tract. (B3, C3, F2, and G2) Splanchnic mesoderm, right and left ventricle. (B4 and C4) Inflow tract. Arrows and arrowheads in whole mounts and sections of Isl1 in situ hybridizations indicate the reduced expression in splanchnic mesoderm and outflow tract.