Fig. 2.

MyD88I inhibits induction of CD80 expression by hBD-3. PBMCs were cultured overnight in medium alone or in medium supplemented with hBD-3 (20 μg/ml), LPS (20 ng/ml), or IFN-α (1,000 units/ml). MyD88I (50 μM) or AG490 (50 μM) was added 18 or 1 h before stimulants, respectively. CD14⁺ cells were evaluated for surface expression of CD80. (A) Expression of CD80 is shown for CD14⁺ cells incubated with hBD-3 (Left) or LPS (Right) in the presence or absence of AG490. (B) Expression of CD80 is shown for CD14⁺ cells incubated with hBD-3, LPS, or IFN-α in the presence or absence of MyD88I. (C) Mean percent inhibition of CD80 induction by AG490 or MyD88I among cells treated with hBD-3, LPS, or IFN-α in four experiments. Error bars represent the standard error of the mean.