Fig. 3.
Effects of ZIP4 on pancreatic cancer growth in the nude mouse model of s.c. xenograft. (A) MIA-ZIP4 or MIA-V cells (3 × 106) were s.c. inoculated into the right flank of nude mice (n = 10 per treatment group). Tumor size was measured weekly for 6 weeks. Tumor volume was calculated by the formula: tumor volume [mm3] = (length [mm]) × (width [mm])2 × 0.52. *, P < 0.01. A representative s.c. tumor mass from each group was shown in the Insets. (B) The s.c. tumors were removed and processed for immunohistochemistry analysis. A monoclonal Ab against Ki67 was used to stain the tissue slides from MIA-V and MIA-ZIP4 groups. s.c. tumors from MIA-ZIP4 group showed much increased cell proliferation by Ki67 staining compared with that of the MIA-V mice. (C) Zinc concentration in nude mouse s.c. tumors. The s.c. tumors were removed and homogenized for zinc detection with ICPMS. The average zinc concentration in the MIA-V group and MIA-ZIP4 group was presented. *, P < 0.05.