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IFN-γ-producing cells after IV challenge with SHIV89.6p virus. Samples were taken 12 weeks after challenge. PBMCs were isolated by a standard Percoll separation technique and assessed for a gag antigen-specific response by ELISpot. The data represent the number of cells able to secrete IFN-γ after SIVgag in vitro stimulation of PBMCs isolated from vaccine-naïve macaques, pCSIVgag-vaccinated, and pCSIVgag- and pmacIL15-immunized macaques.
