Fig. 1.
Increased production of MDA in Huh7-K2040 cells incubated in medium containing delipidated FCS and arachidonate. On day 0, Huh7 (blue) or Huh7-K2040 (red) cells were set up at 7 × 105 cells per 60-mm dish. On day 1, cells were switched to medium supplemented with 10% of FCS or delipidated FCS in the presence or absence 0.3 μM vitamin E or 0.1 mM arachidonate that is conjugated with BSA as indicated. Sixteen hours later, on day 2, cells were harvested, and the amount of MDA in cells was determined as described in Materials and Methods. Values (mean ± SD) from three independent experiments are presented.