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. 2007 Nov 14;104(47):18700–18705. doi: 10.1073/pnas.0704472104

Fig. 5.

Fig. 5.

Multidifferentiation capacity of myoSP. (A). Induction of osteocyte differentiation of myoSP, but not myoMP, as determined by alkaline phosphatase staining (Upper) and by RT-PCR for the expression of osteoblast lineage-specific genes (Lower). (Scale bar, 500 μm.) (B) Relative mRNA expression of the indicated osteocyte markers in myoSP, myoMP, and whole myometrial tissues was examined by semiquantitative RT-PCR and normalized for GAPDH expression. Each bar indicates the mean + SEM of the relative expression ratio obtained from three independent experiments using three individual samples. *, P < 0.05, versus myoMP. (C) Induction of adipocyte differentiation of myoSP, but not myoMP, as determined by Oil red-O staining (Upper) and by RT-PCR for the expression of adipocyte lineage-specific genes (Lower). (Scale bar, 250 μm.) (D) Each bar indicates the mean + SEM of the relative mRNA expression of the adipocyte markers from three independent experiments using three individual samples. *, P < 0.05, versus myoMP. Induction (−) and (+) indicate the 21-day treatment with control media and osteocyte- (C) or adipocyte- (D) inducing media, respectively.