Table 1.

Substrate specificity of the recombinant SbNIT4A enzyme

Substrate	Specific activity, nkat (mg protein)−1
Pentanenitrile	0.32 ± 0.12
Indole-3-acetonitrile	0.55 ± 0.06
4-Hydroxymandelonitrile*	0.57 ± 0.23
5-Hexenenitrile	0.70 ± 0.07
3-Butenenitrile	0.76 ± 0.07
6-Heptenenitrile	1.03 ± 0.06
3-Phenylpropionitrile	1.32 ± 0.36
4-Phenylbutyronitrile	2.50 ± 1.08
2-Phenylacetonitrile	15.68 ± 1.60
4-Hydroxyphenylacetonitrile	27.13 ± 5.68

Shown are the means ± SD from three independent experiments performed in triplicate.

*Measured at pH 6.0 because the compound is labile at pH 8.0.