Fig. 1.

Proviral integration sites in the TERT and myb gene. (A) Schematic view of proviral integration sites and predicted transcription-factor-binding sites in the TERT. The position of identified proviral integration sites in the chTERT promoter/enhancer region are depicted as the number of nucleotides upstream of the TERT transcriptional initiation site. The transcription-factor-binding sites were predicted by Transcription Element Search Software (TESS). The arrows indicate the EcoRI sites, and the black bar shows the location of the probe used in Southern blot analysis. (B) Southern blot analysis was carried out with EcoRI-digested genomic DNA from normal uninfected liver (NL) and bursa (NB), liver tumors, or bursa tumors by using a probe in TERT exon 2 (nt 1,179–2,105). (C) The blot was stripped and reprobed with a probe spanning from myb intron 3 to exon 5. The integrations of proviral sequences introduce additional EcoRI sites and rearrange the WT 5.3-kb alleles to a 4.0-kb band.