Table 1.

ATPase activities and kinetic constants

Construct	Turnover rate,s−1	Vmax, nmol of Pi·min−1·mg−1	Km, mM	n
NBD2-WT	0.08 ± 0.002	60.8 ± 1.8	0.41 ± 0.04	4
NBD2-R1380C	0.09 ± 0.004	70.4 ± 3.1*	0.38 ± 0.05	3
NBD2-R1380L	0.13 ± 0.012	104.3 ± 9.9**	0.55 ± 0.09	3

WT, wild type. Values are given as mean ± SEM for n separate protein preparations. Significance compared with NBD2-WT: *, P < 0.05; **, P < 0.01.