Figure 3.

Disruption of Candida albicans KRE9 gene. (A) Schematic representation of the disruption strategy (see Materials and Methods). (B) Results of the Southern blot verifications of the correct integration of the hisG-URA3-hisG disruption module into the CaKRE9 gene and proper CaURA3 excision after fluoroorotic acid treatment. Extracted genomic DNAs are from CAI4 wild-type cells (lane 1), CaKRE9/cakre9Δ∷hisG-URA3-hisG heterozygous mutant (lane 2), CaKRE9/cakre9Δ∷hisG heterozygous mutant obtained after fluoroorotic acid treatment (lane 3), and a cakre9Δ∷hisG/cakre9Δ:hisG-URA3-hisG homozygous null mutant that was grown on galactose (lane 4).