Figure 4.

Detection of cell wall β-1,6-glucan levels in wild-type and cakre9Δ mutants grown by using galactose as the sole carbon source. A specific anti-β-1,6-glucan antiserum was used to detect whole cell alkali-soluble β-1,6-glucan of different C. albicans strains by Western blotting followed by an enhanced chemiluminescence procedure (see Materials and Methods).