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. 1985 Oct;164(1):192–200. doi: 10.1128/jb.164.1.192-200.1985

Membrane particles from Escherichia coli and Bacillus subtilis, containing penicillin-binding proteins and enriched for chromosomal-origin DNA.

E J Bone, J A Todd, D J Ellar, M G Sargent, A W Wyke
PMCID: PMC214229  PMID: 3930465

Abstract

Rapid-sedimenting DNA-membrane complexes were obtained from both Bacillus subtilis and Escherichia coli by a method involving gentle lysis followed by restriction enzyme digestion and sucrose gradient fractionation. These complexes were substantially enriched in chromosomal origin DNA, and in B. subtilis, the complexes were enriched in penicillin-binding proteins relative to that of the total membrane. Such complexes may represent procaryotic membrane domains which are topographically and functionally distinct.

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Selected References

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