Figure 2.

Cooperation between IRF-3 or its mutants (described in Materials and Methods) and NDV in activation of the IFNA and the IFNB promoters. The experiments were carried out as described in Fig. 1 with IFNA4/CAT reporter (A) or IFNB/CAT reporter (B). (C) Expression of IRF-3 wild type (wt) and its mutants in transfected cells used for CAT assay determined by Western blotting. (D) Two micrograms of IRF-3 was cotransfected with 2 μg of either IFNA4/CAT plasmid or mutant IFNA4/CAT plasmid (mutations are at −94 and −103 nt).