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Phosphorylation of (H89)-C by PDK1 at Thr-197. (A) Phosphorylation of wild-type C, H89-C, and mutant T197D by PDK1. Phosphorylation was analyzed by SDS/PAGE followed by autoradiography. (B) Western blot analysis using antibodies specific for the phosphorylated PKC activation loop. (C) Immunoblot using anti-C subunit antibodies to show equivalent amounts of wild-type C, (H89)-C, and T197D were used in A and B.
