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. 1998 Aug 18;95(17):9861–9866. doi: 10.1073/pnas.95.17.9861

Figure 5.

Figure 5

Supplementation of low levels of GroEL in E. coli by minichaperones. E. coli AI90[pBAD-EL] has the chromosomal groEL gene deleted by P1 transduction (21), and essential GroEL is provided by a plasmid-borne copy of the gene pBAD-EL, which can be tightly regulated by arabinose (30). In the absence of arabinose, the cells are not viable, unless pJCGroEL(1–548) is present, which expresses intact GroEL (○). pJCsht (▵) and pJCGroELsht-(193–335)Y203E (□) plasmids are controls, and cells containing these show increased viability with increasing arabinose concentrations. Overexpression of sht-GroEL(191–376) (•) marginally increases viability; sht-GroEL(191–345) (▴), and (193–335) (■) minichaperones both give significant increases. Each value recorded is the plating efficiency relative to that in the presence of 0.2% arabinose (100%).