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(Upper) Rates (kSH) of Cys 405 sulfhydryl exchange in subunits of procapsid shells at 30°C (•) and expanded shells at 2°C (■). Data were obtained by measuring the intensity decay of the Raman S-H band of the shell as a function of time of exposure to D2O. (Lower) Arrhenius plot showing temperature dependence of the sulfhydryl exchange rate (kSH) in subunits of the procapsid shell. The slope of the plot corresponds to an activation energy for local unfolding (Ealocal) of 44 kcal⋅mol−1.
