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GEF specificity of ARNO/Ch-1-like proteins for ARF1 versus ARF6. Measurement of GTPγS-binding to myrARF1 (□, ■) or myrARF6 (○, •) proteins (1 μM) in the absence (□, ○) or the presence (■, •) of bacterially expressed GEF (40 μg/ml of total soluble E. coli protein). (▵), spontaneous GTPγS binding on myrARF6 in low free Mg2+ concentration (1 μM). (A) ARNO, (B) Ch-1, (C) ARNO3, (D) purified isolated ARNO Sec7 domain (80 nM). Data shown are representative of three independent experiments.
