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Overexpression of mycARNO3 induces fragmentation of the Golgi apparatus and β-COP redistribution. BHK-21 cells were transfected with the mycARNO3 construct and processed for immunofluorescence. Cells were double-labeled with anti-ARNO antibodies (A and E) or anti-myc tag mAb (C and G) in combination with anti-giantin (B), anti-p23 (D), anti-β-COP (F), and anti-sec61β (H). The arrows indicate mycARNO3-expressing cells. (Bar = 15 μm.)
