Figure 5.

mycARNO3 overexpression has no effect on the endocytic pathway. BHK or HeLa (C and D) cells were transfected with mycARNO3. Cells were double-labeled with anti-ARNO3 antibodies to visualize the transfected cells (A, C, and E) in combination with antibodies against the α-adaptin component of plasma membrane AP-2 adaptor complexes (B), to anti-transferrin receptor (Tfn-R)(D), or with phalloidin to visualize F-actin distribution (F). The arrows indicate mycARNO3-expressing cells. (Bar = 15 μm.)