Figure 3.

Protection from apoptosis by caspase inhibitors and insulin. E1.5 embryos were cultured in basal medium alone (Med) or with the indicated concentrations of leupeptin (Leup), caspase 1 and 3 inhibitors (C-Inh), chicken insulin (Ins), or 100 nM insulin and 0.5 mM each caspase inhibitors together. After 10 h of culture, apoptosis was quantified in dissociated cells. Six hundred cells were counted per experimental point. The values represent the mean ± SEM of three to five cultured embryos in each of two to eight different experiments and are expressed relative to the embryos cultured in medium alone. ∗, P < 0.001 with respect to the medium alone.